Bovine IgM Antibody
Bovine IgM Antibody
Product Specifications
Product Name Alternative
Cow immunoglobulin M. IgM whole molecule, Bovine IgM
Conjugation
Unconjugated
Source
Bovine
Origin
Bovine
Field of Research
Protein Biochemistry
Concentration
1.0 mg/mL
Dilution
ELISA: User Optimized, IHC: User Optimized, WB: User Optimized
Purity
Bovine IgM whole molecule was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Bovine IgM whole molecule assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Bovine Serum and anti-Bovine IgM (μ chain specific) . No reaction was observed against anti-Bovine IgG F (c) . Some light chain cross reactivity will occur with anti-Bovine IgG.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at 4° C prior to opening. Bovine IgM whole molecule is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Bovine IgM whole molecule has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Tested Applications
SDS-PAGE
Preservative
Preservative: 0.1% (w/v) Sodium Azide; Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
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