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Bovine IgM Antibody

Bovine IgM Antibody

Product Specifications

Product Name Alternative

Cow immunoglobulin M. IgM whole molecule, Bovine IgM

Conjugation

Unconjugated

Source

Bovine

Origin

Bovine

Field of Research

Protein Biochemistry

Concentration

1.0 mg/mL

Dilution

ELISA: User Optimized, IHC: User Optimized, WB: User Optimized

Purity

Bovine IgM whole molecule was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Bovine IgM whole molecule assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Bovine Serum and anti-Bovine IgM (μ chain specific) . No reaction was observed against anti-Bovine IgG F (c) . Some light chain cross reactivity will occur with anti-Bovine IgG.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at 4° C prior to opening. Bovine IgM whole molecule is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

Bovine IgM whole molecule has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

Tested Applications

SDS-PAGE

Preservative

Preservative: 0.1% (w/v) Sodium Azide; Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0

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