Crasp2 Control protein

Crasp2 is a fusion protein with an MBP tag and was expressed in E. coli. Analysis by SDS-PAGE resulted in a pattern consistent with purified Crasp2 and was estimated to be greater than 90% pure.

Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.

Crasp2 is suitable as a control in immunological assays. Specific conditions for reactivity should be optimized by the end user. Expect bands at 67.8 kDa for CRASP-2-MBP, (25.4 kDa for CRASP-2 and 42.4 kDa for MBP) and in size corresponding to Crasp2 by Western blotting in the appropriate cell lysate or extract. Complement Regulator-Acquiring Surface Protein 2 was tested in SDS-page and western blot.