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Crasp2 Control protein

Crasp2 Control protein

Product Specifications

Product Name Alternative

Control protein, Borrelia burgdorferi CRASP-2, CRASP2

UniProt

O50665

Conjugation

Unconjugated

Source

Borrelia burgdorferi

Origin

Borrelia burgdorferi

Field of Research

Protein Biochemistry

Concentration

1.0mg/mL

Dilution

ELISA: User Optimized, WB: User Optimized

Purity

Crasp2 is a fusion protein with an MBP tag and was expressed in E. coli. Analysis by SDS-PAGE resulted in a pattern consistent with purified Crasp2 and was estimated to be greater than 90% pure.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Crasp2 is suitable as a control in immunological assays. Specific conditions for reactivity should be optimized by the end user. Expect bands at 67.8 kDa for CRASP-2-MBP, (25.4 kDa for CRASP-2 and 42.4 kDa for MBP) and in size corresponding to Crasp2 by Western blotting in the appropriate cell lysate or extract. Complement Regulator-Acquiring Surface Protein 2 was tested in SDS-page and western blot.

Tested Applications

SDS-PAGE, WB

NCBI Accession Number

WP_010890313.1

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

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