DbpA Control protein
DbpA Control protein
Product Specifications
Product Name Alternative
Decorin-binding Protein A, Borrelia burgdorferi dbpA, control protein, DbpA Control Protein
UniProt
O50917
Conjugation
Unconjugated
Source
Borrelia burgdorferi
Origin
Borrelia burgdorferi
Field of Research
Signal Transduction
Concentration
1.0
Dilution
ELISA: User Optimized, WB: User Optimized
Purity
DbpA is a fusion protein with an MBP tag and was expressed in E. coli. Analysis by SDS-PAGE resulted in a pattern consistent with purified Decorin Binding Protein A and was estimated to be greater than 90% pure.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
DbpA is suitable as a control in immunological assays. Specific conditions for reactivity should be optimized by the end user. Expect bands at 60.9 kDa for DbpA-MBP, (18.5kDa for DbpA and 42.4 kDa for MBP) in size corresponding to DbpA by Western blotting in the appropriate cell lysate or extract. Decorin Binding Protein A has been tested in SDS-page and western blot.
Tested Applications
SDS-PAGE, WB
NCBI Accession Number
WP_010890380.1
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
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