Anti-FKBP52 Antibody

Mouse Monoclonal Antibody specific to FKBP52

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

PPIase FKBP4, EC 5.2.1.8, 51 kDa FK506-binding protein, FKBP51, 52 kDa FK506-binding protein, 52 kDa FKBP, FKBP-52, 59 kDa immunophilin, p59, FK506-binding protein 4, FKBP-4, FKBP59, HSP-binding immunophilin, HBI, Immunophilin FKBP52, Rotamase [Cleaved into: Peptidyl-prolyl cis-trans isomerase FKBP4, N-terminally processed]

Gene Name

FKBP4

Gene ID

2288

NCBI Gene ID

UniProt

Q02790

Accession Number

NP_002005.1

Cellular Locus

Cytoplasm, cytosol, Mitochondrion, Nucleus, Cytoplasm, cytoskeleton, Cell projection, axon

Host

Mouse

Reactivity

Human, Mouse, Rat, Dog, Hamster

Immunogen

Recombinant full-length FKBP52 with an N-terminal polyhistidine tag.

Target Antigen

Peptidyl-prolyl cis-trans isomerase FKBP4

Target

FKBP52

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, IHC, ICC/IF, IP

Field of Research

Heat Shock& Stress Proteins

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Immunophilin protein with PPIase and co-chaperone activities. Component of steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments. The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening. Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly. May have a protective role against oxidative stress in mitochondria. {PubMed:1279700, PubMed:1376003, PubMed:19945390, PubMed:21730050, PubMed:2378870}.

Additionnal Information

Immunoblotting: use at 0.5-1ug/mL. A band of ~52 kDa is detected. Immunohistochemistry: use at 4-5ug/mL Immunoprecipitation: 5ug on 10-20ul Protein A-Sepharose + 100ul sample. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: HeLa cell lysate, rat kidney and rat/mouse testes tissue extracts.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human, mouse, rat, dog, and hamster FKBP52.

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

FK506-binding proteins, FKBP51 and FKBP52, are high molecular weight immunophilins that are part of the mature glucocorticoid receptor heterocomplex. The N-terminal domains of these proteins bind FK506 and have peptidyl-prolyl isomerase activity that converts prolyl peptide bonds within target proteins from cis- to trans- proline. The C-terminal domains contain the tetratricopeptide repeats that are involved in protein-protein interactions with Hsp90. Although FKBP52 and FKBP51 share ~75% sequence similarity, they affect hormone binding by glucocorticoid receptors differently and have different Hsp90-binding characteristics. In addition, FKBP51 is linked to the progesterone receptor while FKBP52 is linked to progesterone, androgen, and glucocorticoid receptors.