Recombinant Blomia tropicalis Paramyosin, partial

Product Specifications

Product Name Alternative

(allergen Blo t 11)

Abbreviation

Recombinant Blomia tropicalis Paramyosin protein, partial

UniProt

Q8MUF6

Expression Region

261-548aa

Organism

Blomia tropicalis (Mite)

Target Sequence

EVELESLKVQLEEESEARLELERQLTKANGDAASWKSKYEAELQAHVDEVEELRRKMAQKISEYGEQLEALLNKCSALEKQKARLQSEVEVLIMDLEKATAHAQALEKRVSQLEKINLDLKSKLEEVSMLLEQTQKDLRVKIADLQKLQHEYEKLRDQKEALARENKKLADDLAEAKSQLNDAHRRIHEQEIEIKRLENEREELAAAYKEAETLRKQEEAKNQRLTAELAQTRHDYEKRLAQKEEEIEALRKQYQIEIEQLNMRLAEAEAKLKTEVARLKKKYQAQIT

Tag

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Type

Developed Protein

Source

E.coli

Field of Research

Others

Relevance

Paramyosin is a major structural component of many thick filaments isolated from invertebrate muscles.

Endotoxin

Not test

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Molecular Weight

41.2 kDa

References & Citations

"cDNA cloning and expression of Blo t 11, the Blomia tropicalis allergen homologous to paramyosin." Ramos J.D., Cheong N., Lee B.W., Chua K.Y., Nge C., Wah L.B., Yan C.K. Int. Arch. Allergy Immunol. 126:286-293 (2001)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.