GZMA Antibody

Goat polyclonal antibody to Granzyme A

Product Specifications

Product Name Alternative

Anti-GRAA_HUMAN antibody, anti-Granzyme 1 cytotoxic T lymphocyte associated serine esterase 3 antibody, anti-Granzyme A (Cytotoxic T lymphocyte associated serine esterase 3; Hanukah factor serine protease) antibody, anti-Granzyme A (granzyme 1, cytotoxic T lymphocyte associated serine esterase 3) antibody, anti-Granzyme A antibody, anti-Granzyme A precursor antibody, anti-Granzyme-1 antibody, anti-GZMA antibody

Target

GZMA

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

QLTEKAKINKY

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:8000, WB: 2 μg/ml

Molecular Weight

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Preliminary experiments gave bands at approx 37kDa and 20kDa in lysates of cel line Jurkat after 2 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 29.0kDa according to NP_006135.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Prediction Reactivity

Human

Tested Applications

ELISA

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.