NTE Antibody

Goat polyclonal antibody to PNPLA6

Product Specifications

Product Name Alternative

Anti PNPLA6 antibody, anti patatin-like phospholipase domain containing 6 antibody, anti NTEMND antibody, anti SPG39 antibody, anti sws antibody, anti neuropathy target esterase antibody

Target

NTE

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

YQGRQTSSIFEAAKQ

Field of Research

Epigenetics

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:64000, WB: 2 μg/ml

Molecular Weight

146

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Preliminary experiments gave an approx 75kDa band in lysates of cell lines HeLa and Kelly after 2 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 146kDa according to NP_006693.3. The 75kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Prediction Reactivity

Human, Mouse

Tested Applications

ELISA

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.