Goat anti-MTHFR Antibody
Goat polyclonal antibody to MTHFR
Product Specifications
Product Name Alternative
Anti MTHFR antibody, anti 5,10-methylenetetrahydrofolate reductase (NADPH) antibody, anti OTTHUMP00000002367 antibody, anti OTTHUMP00000002368 antibody, anti methylenetetrahydrofolate reductase intermediate form antibody
Reactivity
Human
Target
MTHFR
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
NRPTQNARETEAP
Field of Research
Metabolism Research; Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:16000, WB: 0.2 μg/ml, IHC-P: 3.75 μg/ml
Molecular Weight
74.6
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave an approx 28kDa band in Human Brain (Cerebellum), Skeletal Muscle and Placenta lysates after 0.2 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 74.6kDa according to NP_005948.3. The 28kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Tested Applications
ELISA, IHC
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
4524
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