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UPF3A/RENT3A Antibody

Goat polyclonal antibody to UPF3A

Product Specifications

Product Name Alternative

Anti UPF3A antibody, anti UPF3 regulator of nonsense transcripts homolog A (yeast) antibody, anti HUPF3A antibody, anti RENT3A antibody, anti UPF3 antibody, anti OTTHUMP00000018789 antibody, anti UPF3 regulator of nonsense transcripts homolog A antibody

Target

UPF3A / RENT3A

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

KETDKQKKIAEKE

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:8000, WB: 0.3 μg/ml

Molecular Weight

54.7; 51.0

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Preliminary experiments gave an approx 48kDa band in lysates of cell lines HeLa and Kelly after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 51kDa according to NP_542418.1 and the reported sizes of 65 to 70kDa from different sources. The 48kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Prediction Reactivity

Bovine, Human

Tested Applications

ELISA

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

65110

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