UPF3A/RENT3A Antibody
Goat polyclonal antibody to UPF3A
Product Specifications
Product Name Alternative
Anti UPF3A antibody, anti UPF3 regulator of nonsense transcripts homolog A (yeast) antibody, anti HUPF3A antibody, anti RENT3A antibody, anti UPF3 antibody, anti OTTHUMP00000018789 antibody, anti UPF3 regulator of nonsense transcripts homolog A antibody
Target
UPF3A / RENT3A
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
KETDKQKKIAEKE
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:8000, WB: 0.3 μg/ml
Molecular Weight
54.7; 51.0
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Preliminary experiments gave an approx 48kDa band in lysates of cell lines HeLa and Kelly after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 51kDa according to NP_542418.1 and the reported sizes of 65 to 70kDa from different sources. The 48kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Prediction Reactivity
Bovine, Human
Tested Applications
ELISA
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
65110
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