SATB1 Antibody
Goat polyclonal antibody to SATB1
Product Specifications
Product Name Alternative
Anti SATB1 antibody, anti SATB homeobox 1 antibody, anti DNA-binding protein SATB1 antibody, anti special AT-rich sequence binding protein 1 antibody, anti special AT-rich sequence binding protein 1 (binds to nuclear matrix/scaffold-associating DNA\1s) antibody
Reactivity
Human
Target
SATB1
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
ESDEENRQKTRPRT
Field of Research
Epigenetics & Chromatin
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:32000, WB: 0.3-1 μg/ml, IHC-P: 3-5μg/ml
Molecular Weight
86.0. 89.0; 78.3
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
IHC: In paraffin embedded Human Thymus shows select nuclear staining. Recommended concentration, 3-5μg/ml.WB: Experiments gave bands at approx. 110kDa and 85kDa in lysates of cell line MOLT4 after 0.3 μg/ml antibody staining. The observed 110kDa band corresponds to earlier findings in literature with different antibodies (De Belle et al, J Cell Biol. 1998 Apr 20;141 (2) :335-48.; PMID: 9548713) . This protein has a calculated MW of 86.0kDa according to NP_002962.1. Recommended concentration: 0.3-1 μg/ml.
Prediction Reactivity
Bovine, Mouse, Rat
Tested Applications
ELISA, FC, IF, IHC, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
6304
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