Neurogranin precursor Antibody
Goat polyclonal antibody to NRGN
Product Specifications
Product Name Alternative
Anti NRGN antibody, anti neurogranin (protein kinase C substrate, RC3) antibody, anti RC3 antibody, anti hng antibody, anti calmodulin-binding protein antibody, anti neurogranin antibody, anti protein kinase C substrate antibody
Reactivity
Human
Target
Neurogranin precursor
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
KIKSGERGRKG
Field of Research
Neuroscience
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:8000, WB: 0.1-0.3 μg/ml, IHC-P: 5 μg/ml
Molecular Weight
7.62
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 15kDa band observed in Human Brain (Cerebral Cortex and Hippocampus), and in Mouse Brain and Rat Brain lysates (calculated MW of 7.6kDa according to NP_006167.1) . There is also an additional 16kDa band. The observed molecular weights correspond to earlier findings in literature with different antibodies (Li et al, J Biol Chem. 1999 Jan 15;274 (3) :1294-300; PMID: 9880498) . Recommended concentration: 0.1-0.3 μg/ml.
Prediction Reactivity
Bovine, Canine, Mouse
Tested Applications
ELISA, IHC, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
4900
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