Anti-ALKBH3 Antibody

Boster Bio Anti-ALKBH3 Antibody catalog # A06750. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3 ;1.14.11.54

Gene Name

ALKBH3

UniProt

Q96Q83

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant full length Human ALKBH3.

Clonality

Polyclonal

Tissue Specificity

Ubiquitous. Detected in heart, pancreas, skeletal muscle, thymus, testis, ovary, spleen, prostate, small intestine, peripheral blood leukocytes, urinary bladder and colon.

Applications

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Dilution

WB: 1:500-1:2000

Form

Liquid

Function

Dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A) (PubMed:12486230, PubMed:12594517, PubMed:16174769, PubMed:26863196, PubMed:26863410) . Repairs alkylated DNA containing 1- methyladenosine (m1A) and 3-methylcytosine (m3C) by oxidative demethylation (PubMed:12486230, PubMed:12594517, PubMed:16174769, PubMed:25944111) . Has a strong preference for single-stranded DNA (PubMed:12486230, PubMed:12594517, PubMed:16174769) . Able to process alkylated m3C within double-stranded regions via its interaction with ASCC3, which promotes DNA unwinding to generate single-stranded substrate needed for ALKBH3 (PubMed:22055184) . Also acts on RNA (PubMed:12594517, PubMed:16174769, PubMed:26863196, PubMed:26863410, PubMed:16858410) . Demethylates N (1) -methyladenosine (m1A) RNA, an epigenetic internal modification of messenger RNAs (mRNAs) highly enriched within 5'- untranslated regions (UTRs) and in the vicinity of start codons (PubMed:26863196, PubMed:26863410) . Requires molecular oxygen, alpha-ketoglutarate and iron (PubMed:22055184, PubMed:16858410) .