Anti-TOPBP1 Antibody

Boster Bio Anti-TOPBP1 Antibody catalog # A01867. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

DNA topoisomerase 2-binding protein 1; DNA topoisomerase II-beta-binding protein 1; TopBP1; DNA topoisomerase II-binding protein 1; TOPBP1; KIAA0259;

Gene Name

TOPBP1

UniProt

Q92547

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide of human TOPBP1 (NP_008958.2) .

Clonality

Polyclonal

Tissue Specificity

Highly expressed in heart, brain, placenta, lung and kidney.

Applications

WB, IHC

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Form

Liquid

Function

Required for DNA replication. Plays a role in the rescue of stalled replication forks and checkpoint control. Binds double- stranded DNA breaks and nicks as well as single-stranded DNA. Recruits the SWI/SNF chromatin remodeling complex to E2F1- responsive promoters. Down-regulates E2F1 activity and inhibits E2F1-dependent apoptosis during G1/S transition and after DNA damage. Induces a large increase in the kinase activity of ATR (PubMed:16530042) .

Molecular Weight

170679 MW

Shipping Conditions

Available

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Other Gene Names

DNA topoisomerase 2-binding protein 1

Subcellular Location

Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Chromosome. Detected on unpaired autosomes in meiotic prophase cells. Detected on X and Y chromosomes during later stages of prophase. Colocalizes with ATR and H2AFX at unsynapsed chromosome cores during prophase (By similarity) . Has a uniform nuclear distribution during G phase. Colocalizes with BRCA1 at stalled replication forks during S phase. In mitotic cells it colocalizes with BRCA1 at spindle poles and centrosomes during metaphase and anaphase. Detected in discrete foci together with PML and numerous DNA repair enzymes after DNA damage by alkylating agents, UV or gamma irradiation. Localizes to sites of DNA damage in a H2AX- independent manner. .