Goat anti-PCDH11Y / PCDH11X Antibody
Goat polyclonal antibody to PCDH11Y
Product Specifications
Product Name Alternative
Anti PCDH11Y antibody, anti protocadherin 11 Y-linked antibody, anti PCDH-PC antibody, anti PCDH11X antibody, anti PCDH22 antibody, anti PCDHY antibody, anti OTTHUMP00000033077 antibody, anti protocadherin 11 X-linked antibody, anti protocadherin 22 antibody, anti protocadherin Y antibody, anti RP13-138P15.1 antibody, anti PCDH-X antibody, anti PCDH11 antibody, anti PCDHX antibody, anti protocadherin 11X antibody, anti protocadherin X antibody, anti protocadherin-S antibody
Reactivity
Human
Target
PCDH11Y / PCDH11X
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
KYNWVTTPTTFK
Field of Research
Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:8000, IHC-P: 6μg/ml
Molecular Weight
115; 116; 147; 113; 113; 148
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Preliminary experiments gave an approx 75kDa band in Human Brain (Amygdala, Hippocampus, Substantia nigra) lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 115kDa according to NP_116753.1 and of 113kDa according to NP_055337.1, the smallest reported isoforms. The 75kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Tested Applications
ELISA, IHC
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
27328, 83259
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