Anti-MiTF Antibody

Boster Bio Anti-MiTF Antibody catalog # A00269-2. Tested in WB, ICC, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

Microphthalmia-associated transcription factor; Class E basic helix-loop-helix protein 32; bHLHe32; MITF; BHLHE32;

Gene Name

MITF

UniProt

O75030

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein

Clonality

Polyclonal

Tissue Specificity

Isoform M is exclusively expressed in melanocytes and melanoma cells. Isoform A and isoform H are widely expressed in many cell types including melanocytes and retinal pigment epithelium (RPE) . Isoform C is expressed in many cell types including RPE but not in melanocyte-lineage cells. Isoform Mdel is widely expressed in melanocytes, melanoma cell lines and tissues, but almost undetectable in non-melanoma cell lines.

Applications

WB, ICC, Flow Cytometry

Purification

ProA affinity purified

Form

Liquid

Function

Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to M-boxes (5'-TCATGTG-3') and symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR) . Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1) . Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.