Goat anti-COL4A6 Antibody
Goat polyclonal antibody to Collagen IV
Product Specifications
Product Name Alternative
COL4A6; collagen, type IV, alpha 6; MGC88184; OTTHUMP00000023834; OTTHUMP00000023835; collagen IV, alpha-6 polypeptide; collagen alpha 6 type IV; collagen of basement membrane, alpha-6; dJ889N15.4 (Collagen Alpha 6 (IV) ) ; type IV alpha 6 collagen
Reactivity
Human
Target
COL4A6
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
PSPEFETETLHNKES
Field of Research
Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:32000, IHC-P: 10ug/ml
Molecular Weight
164; 157; 161;158
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
This antibody is expected to recognize both reported isoforms (NP_001838.2; NP_378667.1) .ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx 20kDa band in Human Heart, Kidney and Skeletal Muscle lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 164kDa according to NP_001838.2 and NP_378667.1. The 20kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Tested Applications
ELISA, FC, IF
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
1288
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