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Goat anti-COL4A6 Antibody

Goat polyclonal antibody to Collagen IV

Product Specifications

Product Name Alternative

COL4A6; collagen, type IV, alpha 6; MGC88184; OTTHUMP00000023834; OTTHUMP00000023835; collagen IV, alpha-6 polypeptide; collagen alpha 6 type IV; collagen of basement membrane, alpha-6; dJ889N15.4 (Collagen Alpha 6 (IV) ) ; type IV alpha 6 collagen

Reactivity

Human

Target

COL4A6

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

PSPEFETETLHNKES

Field of Research

Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:32000, IHC-P: 10ug/ml

Molecular Weight

164; 157; 161;158

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

This antibody is expected to recognize both reported isoforms (NP_001838.2; NP_378667.1) .ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx 20kDa band in Human Heart, Kidney and Skeletal Muscle lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 164kDa according to NP_001838.2 and NP_378667.1. The 20kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Tested Applications

ELISA, FC, IF

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

1288

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