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Goat anti-ICOS Antibody

Goat polyclonal to ICOS

Product Specifications

Product Name Alternative

Anti ICOS antibody, anti inducible T-cell co-stimulator antibody, anti AILIM antibody, anti CD278 antibody, anti MGC39850 antibody, anti activation-inducible lymphocyte immunomediatory molecule antibody, anti inducible costimulator antibody

Reactivity

Human

Target

ICOS

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

KGGQILCDLTKTKGS

Field of Research

Immunology & Inflammation; Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:32000, WB: 0.01 μg/ml, IHC-P: 3.75 μg/ml

Molecular Weight

22.6

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx 13kDa band in Human Peripheral Blood Lymphocyte lysates after 0.01 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 22.6kDa according to NP_036224.1. The 13kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Tested Applications

ELISA, IHC

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

29851

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