NEBL/nebulette Antibody
Goat polyclonal antibody to NEBL
Product Specifications
Product Name Alternative
Anti NEBL antibody, anti nebulette antibody, anti FLJ53769 antibody, anti LNEBL antibody, anti MGC119746 antibody, anti MGC119747 antibody, anti bA56H7.1 antibody, anti LIM-nebulette antibody, anti NEBL, actin-binding Z-disc protein antibody, anti OTTHUMP00000019268 antibody, anti actin-binding Z-disk protein antibody
Target
NEBL / nebulette
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
ELQRLKRTQE
Field of Research
Musculoskeletal & Connective Tissue Research
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:4000, WB: 1 μg/ml
Molecular Weight
116, 31.2
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Preliminary experiments gave an approx 30kDa band in Human Brain (Frontal Cortex) lysates, but not in Human, Mouse and Rat Heart lysates after 1 μg/ml antibody staining. This protein has a calculated MW of 31.2kDa according to NP_998734.1. The 30kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates
Prediction Reactivity
Canine, Human, Mouse
Tested Applications
ELISA
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
10529
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