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NEBL/nebulette Antibody

Goat polyclonal antibody to NEBL

Product Specifications

Product Name Alternative

Anti NEBL antibody, anti nebulette antibody, anti FLJ53769 antibody, anti LNEBL antibody, anti MGC119746 antibody, anti MGC119747 antibody, anti bA56H7.1 antibody, anti LIM-nebulette antibody, anti NEBL, actin-binding Z-disc protein antibody, anti OTTHUMP00000019268 antibody, anti actin-binding Z-disk protein antibody

Target

NEBL / nebulette

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

ELQRLKRTQE

Field of Research

Musculoskeletal & Connective Tissue Research

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:4000, WB: 1 μg/ml

Molecular Weight

116, 31.2

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Preliminary experiments gave an approx 30kDa band in Human Brain (Frontal Cortex) lysates, but not in Human, Mouse and Rat Heart lysates after 1 μg/ml antibody staining. This protein has a calculated MW of 31.2kDa according to NP_998734.1. The 30kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates

Prediction Reactivity

Canine, Human, Mouse

Tested Applications

ELISA

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

10529

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