FITC-Labeled Human Mesothelin / MSLN (296-580) Protein, Fc Tag

FITC-Labeled Human Mesothelin (296-580), Fc Tag (Cat. No. MSN-HF253) is expressed from human HEK293 cells. It contains AA Glu 296 - Gly 580 (Accession # AAH09272.1) .

Product Specifications

Background

Mesothelin (MSLN) is also known as CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, which belongs to the mesothelin family. Mesothelin / MSLN can be proteolytically cleaved into the following two chains by a furin-like convertase: Megakaryocyte-potentiating factor (MPF) and the cleaved form of mesothelin. Both MPF and the cleaved form of mesothelin are N-glycosylated. Mesothelin / MSLN can interacts with MUC16. The membrane-anchored forms of MSLN may play a role in cellular adhesion. MPF potentiates megakaryocyte colony formation in vitro.

Specifications

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 58.62 kDa. The protein migrates as 60-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

Q13421-3 (R309P)

Expression Region

Glu 296 - Gly 580

Host

HEK293

Target

Mesothelin

Conjugation

FITC Labeled

Tag

C-Fc

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Anti-Human MSLN MAb at 10 μg/mL (100 μL/well) can bind FITC-Labeled Human Mesothelin (296-580), Fc Tag (Cat. No. MSN-HF253) with a linear range of 0.078-0.625 μg/mL (Ex.488 nm/Em.535 nm) (QC tested).