DTME

DTME (dithio-bis-maleimidoethane) is a homobifunctional, maleimide crosslinker specifically designed for conjugation between sulfhydryl groups (-SH) . DTME, whose molecular structure consists of two maleimide groups connected by an ethylene disulfide bridge, can specifically react with thiol - containing molecules (such as cysteine residues) to form stable covalent bonds. DTME allows crosslinks that can be cleaved with reducing agents such as DTT (HY-15917) . DTME is commonly utilized to explore and characterize protein structure, particularly oligomerization, or protein interactions[1].

Product Specifications

CAS Number

[71865-37-7]

Product Name Alternative

Dithio-bis-maleimidoethane

UNSPSC

12352005

Hazard Statement

H302+H312+H332, H315, H319, H335

Target

Biochemical Assay Reagents

Type

Reference compound

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/dtme.html

Concentration

10mM

Purity

99.03

Solubility

DMSO : 50 mg/mL (ultrasonic)

Smiles

O=C(C=C1)N(CCSSCCN2C(C=CC2=O)=O)C1=O

Molecular Formula

C12H12N2O4S2

Molecular Weight

312.36

Precautions

H302+H312+H332, H315, H319, H335

References & Citations

[1]Kovalenko OV, et al. Evidence for specific tetraspanin homodimers: inhibition of palmitoylation makes cysteine residues available for cross-linking. Biochem J. 2004 Jan 15;377 (Pt 2) :407-17.