DEPDC4 Antibody
Goat polyclonal to DEPDC4
Product Specifications
Product Name Alternative
Anti DEPDC4 antibody, anti DEP domain containing 4 antibody, anti DEP.4 antibody, anti FLJ33505 antibody
Target
DEPDC4
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
EPPVKTQNLQLN
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:8000, WB: 1 μg/ml
Molecular Weight
33.7
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Preliminary experiments gave an approx 75kDa band in Human Brain (Amygdala) lysates and in lysates of cell lines A431 and KELLY after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 33.7kDa according to NP_689530.1. The 75kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Prediction Reactivity
Human
Tested Applications
ELISA
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
120863
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