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TRC8 Antibody

Goat polyclonal antibody to RNF139

Product Specifications

Product Name Alternative

Anti RNF139 antibody, anti ring finger protein 139 antibody, anti TRC8 antibody, anti HRCA1 antibody, anti MGC31961 antibody, anti RCA1 antibody, anti patched related protein translocated in renal cancer antibody, anti multiple membrane spanning receptor TRC8 antibody

Reactivity

Human

Target

TRC8

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

HTGAAAEEFNDDT

Field of Research

Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:16000, WB: 0.3-1 μg/ml, IHC-P: 3-4μg/ml

Molecular Weight

76

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.IHC: In paraffin embedded Human Placenta shows membranous staining in the cytotrophoblasts. Recommended concentration, 3-4μg/ml.WB: Experiments gave bands at approx. 150kDa and 50kDa in Human Skeletal Mucle lysate after 0.5 μg/ml antibody staining. This protein has a calculated MW of 76.0kDa according to NP_009149.2.The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.5-1.5 μg/ml.

Prediction Reactivity

Canine

Tested Applications

ELISA, IHC, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

11236

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