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ATG4D Antibody

Goat polyclonal antibody to ATG4D

Product Specifications

Product Name Alternative

Anti ATG4D antibody, anti ATG4 autophagy related 4 homolog D (S. cerevisiae) antibody, anti APG4-D antibody, anti APG4D antibody, anti AUTL4 antibody, anti APG4 autophagy 4 homolog D antibody, anti AUT-like 4, cysteine endopeptidase antibody, anti autophagin-4 antibody, anti autophagy-related cysteine endopeptidase 4 antibody, anti cysteine protease involved in autophagy antibody

Reactivity

Human

Target

ATG4D

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

CSQLAQPTLRLPRT

Field of Research

Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:32000, WB: 1 μg/ml, IHC-P: 2.5 μg/ml

Molecular Weight

52.9

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx. 26kDa band in Human Skeletal Muscle lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 52.9kDa according to NP_116274.3. The 26kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Prediction Reactivity

Canine, Mouse, Porcine, Rat

Tested Applications

ELISA, IHC

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

84971

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