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LMX1B Antibody

Goat polyclonal to LMX1B

Product Specifications

Product Name Alternative

Anti LMX1B antibody, anti LIM homeobox transcription factor 1, beta antibody, anti LMX1.2 antibody, anti MGC138325 antibody, anti MGC142051 antibody, anti NPS1 antibody, anti LIM homeo box transcription factor 1, beta antibody

Reactivity

Human

Target

LMX1B

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

QQALTTSCYFRDRK

Field of Research

Stem Cell & Developmental Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:64000, WB: 0.3 μg/ml, IHC-P: 2.5 μg/ml

Molecular Weight

41.6

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Preliminary experiments gave an approx 100kDa band in Mouse Kidney lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 41.6kDa according to Human NP_002307.1 and to Mouse NP_034855.1. The 100kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Prediction Reactivity

Mouse, Rat

Tested Applications

ELISA, IHC

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

4010

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