Recombinant Human H2AX Protein (Trx Tag)
Product Specifications
Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. Human Histone H2AX with an N-terminal His tag expressed in E. coli and purified by conventional chromatography.
Abbreviation
H2AX
Synonyms
H2A.X; H2A/X; H2AX; Histone H2AX; H2AFX; gamma H2A.X; γH2AX
UniProt
P16104
Accession Number
P16104
Expression System
E.coli
Tag
N-Trx
Sequence
Met1-Tyr143
Field of Research
Epigenetics and Nuclear Signaling
Endotoxin
< 10 EU/mg of the protein as determined by the LAL method
Purity
> 90% as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis
Shipping Conditions
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Calculated Molecular Weight
35.7 kDa
Observed Molecular Weight
35 kDa
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
Species
Human
Available Sizes
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