Human Siglec-10 Protein, Fc Tag
Human Siglec-10, Fc (L118A, G120A, E201A) Tag is expressed from human 293 cells (HEK293) . It contains AA Met 17 - Thr 546 (Accession # Q96LC7-1) .
Product Specifications
UniProt
Q96LC7
Expression Region
Met 17 - Thr 546
Reactivity
Human
Conjugation
Unconjugated
Tag
C-Fc (A3)
Field of Research
Immunology & Inflammation
Endotoxin
1.0 EU per μg
Purity
85%
Form
Powder
Molecular Weight
84.6 kDa
Storage Conditions
-20°C
Notes
For research use only.
Applications Notes
This protein carries a human IgG1 Fc (L118A, G120A, E201A) tag at the C-terminus. The protein has a calculated MW of 84.6 kDa. As the result of the mutations for Fc-Tag, the protein migrates as 90-110 kDa (Siglect-10 with Fc-Tag), 60-67 kDa (Siglect-10) and 30-32 kDa (Fc fragment) under reducing (R) condition (SDS-PAGE) due to glycosylation. Mutations (L118A, G120A, E201A /EU number: L235A/G237A/E318A) in human immunoglobulin G1 (hIgG1) Fc strongly reduce binding of the Fc mutant to cell expressed FcγRs, resulting in an almost 4-fold reduction in ADCC compared to that of wild type human IgG1.
NCBI Accession Number
Q96LC7
Preservative
PBS, pH 7.4
Available Sizes
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