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Cytokeratin 20 Peptide

Cytokeratin 20 Peptide

Product Specifications

Product Name Alternative

KRT20, keratin 20, CK-20, CK20, K20, KRT21, MGC35423, OTTHUMP00000164518, cytokeratin 20, cytokeratin-20, keratin, type I cytoskeletal 20, keratin-20, protein IT

UniProt

P35900

Form

Lyophilized

Storage Conditions

Store at -20°C. When reconstituted minimize freezing and thawing.

Notes

For research use only.

Applications Notes

Cytokeratin 20 peptide is used for blocking the activity of cytokeratin 20 antibody. The cytokeratin 20 antibody ships at 0.5 mg/mL and the STK36 Peptide ships as a 100 ug pellet. When peptide is reconstituted in 200 uL water, the concentration will be 0.5 mg/mL, identical to that of the antibody. The best starting ratio would be 1:1 (which means molar excess of peptide relative to antibody when identical volumes are mixed) . Mix equal volume of peptide and antibody at the required dilution and leave at ambient temperature for 20 minutes. Then incubate and develop in parallel two blots: one with the antibody and one with the antibody which has been pre-absorbed with the provided peptide.

Tested Applications

Blocking

NCBI Accession Number

NP_061883.1

Preservative

Cytokeratin 20 peptide is supplied as 100 μg of dried peptide. When the peptide is reconstituted in 200 μL water, the concentration will be 0.5 mg/mL.

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