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Goat anti-SODD Antibody

Goat polyclonal antibody to BAG4

Product Specifications

Product Name Alternative

Anti BAG4 antibody, anti BAG-4 antibody, anti SODD antibody, anti BAG-family molecular chaperone regulator-4 antibody, anti silencer of death domains antibody

Reactivity

Human

Target

SODD

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

GNATSDHPNNQDQSS

Field of Research

Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:8000, WB: 0.3-1 μg/ml, IHC-P: 3.75 μg/ml

Molecular Weight

49.6

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx. 60kDa band observed in cell lines HEK293 and HeLa lysates (calculated MW of 49.6kDa according to NP_004865.1) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Takada et al, Mol Cell Biol. 2003 Jun;23 (11) :4026-33.; PMID: 12748303) . Recommended concentration: 0.3-1 μg/ml. An additional band of unknown identity was also consistently observed at 20kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Tested Applications

ELISA, IHC, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

9530

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