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CHKB Antibody

Goat polyclonal antibody to CHKB

Product Specifications

Product Name Alternative

Anti CHKB antibody, anti choline kinase beta antibody, anti CHETK antibody, anti CHKL antibody, anti choline kinase-like antibody, anti choline/ethanolamine kinase antibody

Target

CHKB

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

KGETLSQEEQRKLEED

Field of Research

Signal Transduction

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:2000, WB: 1 μg/ml

Molecular Weight

45.3; 13.5

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:2000.WB: Preliminary experiments gave an approx 38kDa band in Human Skeletal Muscle lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 45.3kDa according to NP_005189.2. The 38kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Prediction Reactivity

Canine, Human

Tested Applications

ELISA

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

1120

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