CHKB Antibody
Goat polyclonal antibody to CHKB
Product Specifications
Product Name Alternative
Anti CHKB antibody, anti choline kinase beta antibody, anti CHETK antibody, anti CHKL antibody, anti choline kinase-like antibody, anti choline/ethanolamine kinase antibody
Target
CHKB
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
KGETLSQEEQRKLEED
Field of Research
Signal Transduction
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:2000, WB: 1 μg/ml
Molecular Weight
45.3; 13.5
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:2000.WB: Preliminary experiments gave an approx 38kDa band in Human Skeletal Muscle lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 45.3kDa according to NP_005189.2. The 38kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Prediction Reactivity
Canine, Human
Tested Applications
ELISA
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
1120
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