Toluidine Blue

Toluidine Blue (Toluidine Blue O) is an alkaline quinonimine dye (vivo dye) with high affinity for acidic tissue components, staining nuclei blue and polysaccharides purple. Toluidine Blue shows heterostaining properties for mast cells, mucins and chondrocytes. Toluidine Blue can stain different components of plant tissues and cells in different colours. Toluidine Blue is also used as a diagnostic aid to identify malignant lesions, such as cancer[1][2][3].

Product Specifications

CAS Number

[92-31-9]

Product Name Alternative

Toluidine Blue O

UNSPSC

12171500

Hazard Statement

H302

Target

Fluorescent Dye

Type

Dye Reagents

Related Pathways

Others

Field of Research

Cancer

Assay Protocol

https://www.medchemexpress.com/toluidine-blue.html

Solubility

DMSO : 20.83 mg/mL (ultrasonic; warming; heat to 80°C) |H2O : 5 mg/mL (ultrasonic; warming; heat to 60°C)

Smiles

CC1=CC2=C([S+]=C3C=C(N(C)C)C=CC3=N2)C=C1N.[Cl-]

Molecular Formula

C15H16ClN3S

Molecular Weight

305.83

Precautions

H302

References & Citations

[1]Sridharan G, et al. Toluidine blue: A review of its chemistry and clinical utility. J Oral Maxillofac Pathol. 2012;16 (2) :251-255.|[2]Puebla-Osorio N, et al. Detection of Infiltrating Mast Cells Using a Modified Toluidine Blue Staining. Methods Mol Biol. 2017;1627:213-222.|[3]O'Brien T P, et al. Polychromatic staining of plant cell walls by toluidine blue O. Protoplasma, 1964, 59 (2) : 368-373.