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Goat anti-Clusterin / ApoJ (mouse) Antibody

Goat polyclonal antibody to CLU

Product Specifications

Product Name Alternative

Anti Clusterin antibody, anti CLU antibody, anti APOJ antibody, anti CLI antibody, anti KUB1 antibody, anti Apolipoprotein J antibody, anti Apo-J antibody

Reactivity

Mouse, Rat

Target

Clusterin / ApoJ (mouse)

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

EKALQEYRRKSRAE

Field of Research

Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:64000, WB: 0.1-1 μg/ml, IHC-P: 10 μg/ml

Molecular Weight

51.6

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx. 37kDa band observed in Mouse Brain and Testis lysates and in Rat Brain and Ovary lysates (calculated MW of 51.6kDa according to Mouse NP_038520.1) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Miyata et al, Circulation. 2001 Sep 18;104 (12) :1407-12.; PMID: 11560857) . Recommended concentration: 0.3-1 μg/ml.

Tested Applications

ELISA, IF, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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