Lok Peptide

10895, 38B.4, CG10895, chk, CHK-2, chk2, Chk2, CHK2, chk2/lok, Dmchk2, DmChk2, DmCHk2, Dmel\CG10895, Dmnk, Dmnk-L, Dmnk-S, Dmnk/DChk2, LOKI/CHK2, mnk, MNKProtein loki

Lok Antibody ships at 0.5 mg/mL and the lok Peptide ships as a 100 μg pellet. When peptide is reconstituted in 200 μL water, the concentration will be 0.5 mg/mL, identical to that of the antibody. The best starting ratio would be 1:1 (which means molar excess of peptide relative to antibody when identical volumes are mixed) . Mix equal volume of peptide and antibody at the required dilution and leave at ambient temperature for 20 minutes. Then incubate and develop in parallel two blots: one with the antibody and one with the antibody which has been pre-absorbed with the provided peptide.