P-Nitrophenyl phosphorylcholine
P-Nitrophenyl phosphorylcholine (4-Nitrophenylphosphorylcholine) is a chromogenic substrate that is used to measure phospholipase C (PLC) activity. Hydrolysis of p-nitrophenylphosphorylcholine by PLC results in the liberation of p-nitrophenol, which can be measured at 405 nm at pH 7.2-7.5.
Product Specifications
CAS Number
[21064-69-7]
Product Name Alternative
4-Nitrophenylphosphorylcholine; 4-Nitrophenylphosphorylcholine; O- (4-Nitrophenylphosphoryl) choline
UNSPSC
12352005
Hazard Statement
H302, H312, H315, H319, H332, H335
Target
Endogenous Metabolite
Type
Reference compound
Related Pathways
Metabolic Enzyme/Protease
Field of Research
Metabolic Disease
Assay Protocol
https://www.medchemexpress.com/p-nitrophenyl-phosphorylcholine.html
Purity
99.61
Solubility
DMSO : 25 mg/mL (ultrasonic; warming; heat to 60°C)
Smiles
O=P(OCC[N+](C)(C)C)([O-])OC1=CC=C([N+]([O-])=O)C=C1
Molecular Formula
C11H17N2O6P
Molecular Weight
304.24
Precautions
H302, H312, H315, H319, H332, H335
References & Citations
[1]Berka, et al. Studies of phospholipase C (heat-labile hemolysin) in Pseudomonas aeruginosa. Infect. Immun. 34 (3), 1071-1074 (1981) .
Shipping Conditions
Blue Ice
Storage Conditions
-20°C (Powder, protect from light)
Scientific Category
Reference compound1
Clinical Information
No Development Reported
Available Sizes
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