ANGPTL3 Antibody

Goat polyclonal antibody to ANGPTL3

Product Specifications

Product Name Alternative

Anti ANGPTL3 antibody, anti angiopoietin-like 3 antibody, anti ANGPT5 antibody, anti angiopoietin 5 antibody

Target

ANGPTL3

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

EDQYKQLNQQHSQ

Field of Research

Metabolism Research

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:8000, WB: 0.3 μg/ml

Molecular Weight

53.6

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Preliminary experiments gave bands at approx 37 kDa and 24 kDa in Human Liver and Kidney lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe in these lysates given the calculated size of 53.6 kDa according to NP_055310.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Prediction Reactivity

Human

Tested Applications

ELISA

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.