Human Coronavirus Nucleocapsid (229E) Recombinant Protein
Human Coronavirus Nucleocapsid (229E) Recombinant Protein
Product Specifications
Product Name Alternative
Human CoV 229E N Protein, Human CoV 229E Nucleocapsid Protein
UniProt
P15130
Tag
N-Term MBP Uncleaved
Source
Bacteria
Field of Research
Infectious Disease & Virology
Concentration
Batch dependent
Purity
Amylose Resin (0.85)
Form
Liquid
Molecular Weight
Mono-Isotopic Mass: 87, 651.64 daltons Average Mass: 87, 705.68 daltons
Storage Conditions
Store at -70 °C. Avoid repeated freeze-thaw cycles.
Notes
For research use only.
NCBI Accession Number
NP_073556.1
Preservative
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.03 % Brij-35
AA Sequence
Native Sequence:MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGDDDDKVPEFLEVLFQGPLGSMATVKWADASEPQRGRQGRIPYSLYSPLLVDSEQPWKVIPRNLVPINKKDKNKLIGYWNVQKRFRTRKGKRVDLSPKLHFYYLGTGPHKDAKFRERVEGVVWVAVDGAKTEPTGYGVRRKNSEPEIPHFNQKLPNGVTVVEEPDSRAPSRSQSRSQSRGRGESKPQSRNPSSDRNHNSQDDIMKAVAAALKSLGFDKPQEKDKKSAKTGTPKPSRNQSPASSQTSAKSLARSQSSETKEQKHEMQKPRWKRQPNDDVTSNVTQCFGPRDLDHNFGSAGVVANGVKAKGYPQFAELVPSTAAMLFDSHIVSKESGNTVVLTFTTRVTVPKDHPHLGKFLEELNAFTREMQQHPLLNPSALEFNPSQTSPATAEPVRDEVSIETDIIDEVNAmino acids M1 – N389 (end) .Residue M404 of the fusion protein is equivalent to M1 of the native enzyme.The MBP tag is located at residues 1 – 367.Protease Cleavage:PreScission (LEVLFQGP) residues 393 - 400
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