TNF-alpha Recombinant Protein
TNF-alpha Recombinant Protein
Product Specifications
Product Name Alternative
DIF, TNFA, TNFSF2, TNF-alpha, Tumor necrosis factor, Cachectin, TNF-a
UniProt
P01375
Source
E. coli
Field of Research
Immunology & Inflammation
Purity
Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg) .
Bioactivity
The ED50 was determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is ≤ 0.05 ng/mL, corresponding to a specific activity of ≥ 2 x 107 units/mg.
Form
Lyophilized
Storage Conditions
The lyophilized TNF-alpha recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted TNF-alpha is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing TNF-alpha recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Notes
For research use only.
NCBI Accession Number
NP_000585.2
AA Sequence
VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIAL
Available Sizes
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