TNF-alpha Recombinant Protein

Product Specifications

Product Name Alternative

DIF, TNFA, TNFSF2, TNF-alpha, Tumor necrosis factor, Cachectin, TNF-a

UniProt

P01375

Source

E. coli

Field of Research

Immunology & Inflammation

Purity

Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg) .

Bioactivity

The ED50 was determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is ≤ 0.05 ng/mL, corresponding to a specific activity of ≥ 2 x 107 units/mg.

Form

Lyophilized

Storage Conditions

The lyophilized TNF-alpha recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted TNF-alpha is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing TNF-alpha recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

Notes

For research use only.

NCBI Accession Number

NP_000585.2

AA Sequence

VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIAL