MMP-2 Recombinant Protein

Product Specifications

Product Name Alternative

CLG4, MONA, CLG4A, TBE-1, MMP-II, 72 kDa type IV collagenase, 72 kDa gelatinase, MMP-2

UniProt

P08253

Source

E. coli

Field of Research

Cardiovascular Research; Musculoskeletal & Connective Tissue Research

Purity

Greater than 90% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg) .

Bioactivity

MMP - 2 activity was measured by its ability to cleave a chromogenic peptide MMP - 2 substrate at room temperature. At an MMP - 2 concentration of 2.5 μg/mL, 50% cleavage was achieved at an incubation time of approximately 25 minutes.

Form

Lyophilized

Storage Conditions

The lyophilized MMP-2 recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted MMP-2 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing MMP-2 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

Notes

For research use only.

NCBI Accession Number

NP_004521.1

AA Sequence

MYNFFPRKPK WDKNQITYRI IGYTPDLDPE TVDDAFARAF QVWSDVTPLR FSRIHDGEAD IMINFGRWEH GDGYPFDGKD GLLAHAFAPG TGVGGDSHFD DDELWTLGEG QVVRVKYGNA DGEYCKFPFL FNGKEYNSCT DTGRSDGFLW CSTTYNFEKD GKYGFCPHEA LFTMGGNAEG QPCKFPFRFQ GTSYDSCTTE GRTDGYRWCG TTEDYDRDKK YGFCPETAMS TVGGNSEGAP CVFPFTFLGN KYESCTSAGR SDGKMWCATT ANYDDDRKWG FCPDQGYSLF LVAAHEFGHA MGLEHSQDPG ALMAPIYTYT KNFRLSQDDI KGIQELYGAS PDIDLGTGPT PTLGPVTPEI CKQDIVFDGI AQIRGEIFFF KDRFIWRTVT PRDKPMGPLL VATFWPELPE KIDAVYEAPQ EEKAVFFAGN EYWIYSASTL ERGYPKPLTS LGLPPDVQRV DAAFNWSKNK KTYIFAGDKF WRYNEVKKKM DPGFPKLIAD AWNAIPDNLD AVVDLQGGGH SYFFKGAYYL KLENQSLKSV KFGSIKSDWL GC