Soluble CD14 Recombinant Protein

Biological activity was determined by the dose dependent activation of NF - kappaB in a RAW264 cell line based reporter system, using a sCD14 concentration range of 20 ng/μL to 200 ng/μL. The NF - kappaB activation is enhanced when the assay is done in the presence of 0.25 ng/μL to 1.0 ng/μL bacterial LPS.

The lyophilized Soluble CD14 recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted Soluble CD14 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing Soluble CD14 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

TTPEPCELDD EDFRCVCNFS EPQPDWSEAF QCVSAVEVEI HAGGLNLEPF LKRVDADADP RQYADTVKAL RVRRLTVGAA QVPAQLLVGA LRVLAYSRLK ELTLEDLKIT GTMPPLPLEA TGLALSSLRL RNVSWATGRS WLAELQQWLK PGLKVLSIAQ AHSPAFSCEQ VRAFPALTSL DLSDNPGLGE RGLMAALCPH KFPAIQNLAL RNTGMETPTG VCAALAAAGV QPHSLDLSHN SLRATVNPSA PRCMWSSALN SLNLSFAGLE QVPKGLPAKL RVLDLSCNRL NRAPQPDELP EVDNLTLDGN PFLVPGTALP HEGSMNSGVV P