IL-12 Recombinant Protein

Product Specifications

Product Name Alternative

P35, CLMF, NFSK, NKSF1, IL-12A, Interleukin-12 subunit alpha, Cytotoxic lymphocyte maturation factor 35 kDa subunit

UniProt

P29459

Source

CHO cells

Field of Research

Immunology & Inflammation

Purity

Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg) .

Bioactivity

The ED50 was determined by the induction of IFN - gamma from NK cells co - stimulated with IL - 18 is ≤ 1.0 ng/mL, corresponding to a specific activity of ≥ 1 x 106 units/mg.

Form

Lyophilized

Storage Conditions

The lyophilized IL-12 recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted IL-12 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing IL-12 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

Notes

For research use only.

NCBI Accession Number

NP_000873.2

AA Sequence

P35 Subunit: RNLPVATPDP GMFPCLHHSQ NLLRAVSNML QKARQTLEFY PCTSEEIDHE DITKDKTSTV EACLPLELTK NESCLNSRET SFITNGSCLA SRKTSFMMAL CLSSIYEDLK MYQVEFKTMN AKLLMDPKRQ IFLDQNMLAV IDELMQALNF NSETVPQKSS LEEPDFYKTK IKLCILLHAF RIRAVTIDRV MSYLNAS p40 Subunit: IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCS