ATG5 Antibody
Goat polyclonal antibody to ATG5
Product Specifications
Product Name Alternative
Anti ATG5 antibody, anti ATG5 autophagy related 5 homolog (S. cerevisiae) antibody, anti APG5 antibody, anti APG5L antibody, anti ASP antibody, anti hAPG5 antibody, anti APG5 (autophagy 5, S. cerevisiae) -like antibody, anti APG5 autophagy 5-like antibody, anti ATG5 autophagy related 5-like antibody, anti OTTHUMP00000040507 antibody, anti apoptosis specific protein antibody
Target
ATG5
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
SQVINEMQKKDHKQ
Field of Research
Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:64000, WB: 1 μg/ml
Molecular Weight
32.4
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Preliminary experiments gave an approx. 38 kDa band in NIH3T3 lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 32.4 kDa according to NP_004840.1. The 38 kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Prediction Reactivity
Bovine, Human, Mouse
Tested Applications
ELISA
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
9474
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