Leptin Recombinant Protein

Product Specifications

Product Name Alternative

OB, obese, Ob, Leptin, Obesity factor

UniProt

P50596

Source

E. coli

Field of Research

Metabolism Research

Purity

Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg) .

Bioactivity

Biological activity was determined by an in vivo assay using the ob/ob mouse obesity model and NZO. Both strains of mice were treated via intraperitoneal injection once daily at a dose of 5μg leptin/gm of body weight for 7 days. Significant effects on body weight, food consumption, and plasma glucose levels were observed to saline - treated controls.

Form

Lyophilized

Storage Conditions

The lyophilized Leptin recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted Leptin is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing Leptin recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

Notes

For research use only.

NCBI Accession Number

NP_037208.1

AA Sequence

MVPIHKVQDD TKTLIKTIVT RINDISHTQS VSARQRVTGL DFIPGLHPIL SLSKMDQTLA VYQQILTSLP SQNVLQIAHD LENLRDLLHL LAFSKSCSLP QTRGLQKPES LDGVLEASLY STEVVALSRL QGSLQDILQQ LDLSPEC