Flagellin TLR5 Mutant Recombinant Protein
Flagellin TLR5 Mutant Recombinant Protein
Product Specifications
Product Name Alternative
FliC (TLR5 Mutant)
UniProt
P50591
Tag
His Tag
Source
E. coli
Field of Research
Immunology & Inflammation
Concentration
0.1mg/ml after reconstitution.
Purity
>95% (SDS-PAGE) . Endotoxin level is less than 0.01EU/ μg purified protein (LAL test; Lonza) .
Bioactivity
Does not activate TLR5 in human epithelial cell assays based on NF-kappaB luciferase fusions, in vivo on expression of CCL20 or IL-6. It can be used as negative control for TLR5 signaling assays.
Form
Lyophilized
Storage Conditions
Stable for at least 6 months after receipt when stored at -20°C.
Notes
For research use only.
Applications Notes
This recombinant proteins is for research use only.
NCBI Accession Number
NP_460912
Preservative
Contains PBS. Reconstitute with 100 μl sterile water.
AA Sequence
The native flagellin [FliC] from Salmonella enterica Serovar Typhimurium strain ATCC14028 (aa 1-495) contains a substitution of amino acids 90-97 required for TLR5 signaling by a motif from a non-signaling flagellin and is fused at the N-terminus to a His-tag.
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