SuperKillerTRAIL, Soluble Recombinant Protein
SuperKillerTRAIL, Soluble Recombinant Protein
Product Specifications
Product Name Alternative
TRAIL, Apo-2L, TNFSF10, CD253
UniProt
P10148
Tag
His Tag
Source
E. coli
Field of Research
Cancer Biology, Cell Biology, Immunology & Inflammation, Signal Transduction
Concentration
0.5 mg/ml
Purity
>98% (SDS-PAGE) . Endotoxin level is less than 0.01EU/ μg purified protein (LAL test; Lonza) .
Bioactivity
Induces apoptosis at concentrations of >1ng/ml. Note: Does not require a cross-linking enhancer for its potent biological activity. Shows superior activity on human cell lines that require extensive cross-linking of TRAIL-Rs for killing (e.g. Jurkat) .
Form
Liquid
Storage Conditions
Once thawed it is recommended to prepare appropriate aliquots and to store them at -80°C.
Notes
For research use only.
Applications Notes
This recombinant proteins is for research use only.
NCBI Accession Number
P50591
Preservative
In 20mM HEPES, pH 7.4, 150mM NaCl, 0.005% Tween 20, 1% sucrose, 1mM DTT.
AA Sequence
The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to a His-tag and a linker peptide. The active multimeric conformation is stabilized by an inserted mutation allowing an additional CC-bridge.
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