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SuperKillerTRAIL, Soluble Recombinant Protein

SuperKillerTRAIL, Soluble Recombinant Protein

Product Specifications

Product Name Alternative

TRAIL, Apo-2L, TNFSF10, CD253

UniProt

P13236

Tag

His Tag

Source

E. coli

Concentration

0.5 mg/ml

Purity

>95% (SDS-PAGE) . Endotoxin level is less than 0.01EU/ μg purified protein (LAL test; Lonza) .

Bioactivity

Induces apoptosis at concentrations of >10ng/ml as tested on human tumor cells. This effect can be efficiently blocked by recombinant mouse TRAIL receptors. This protein does not require a cross-linker (enhancer) for its potent biological activity.

Form

Liquid

Storage Conditions

Once thawed it is recommended to prepare appropriate aliquots and to store them at -80°C.

Notes

For research use only.

Applications Notes

This recombinant proteins is for research use only.

NCBI Accession Number

P50592

Preservative

In 20mM HEPES, pH 7.4, 300mM NaCl, 0.01% Tween 20, 1% sucrose, 1mM DTT.

AA Sequence

The extracellular domain of mouse TRAIL (aa 99-291) is fused at the N-terminus to a His-tag and a linker peptide. The active multimeric conformation is stabilized by an inserted mutation allowing an additional CC-bridge.

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